Certificate of HPLC Practice

This course is designed for beginners and intermediate-level users in HPLC who want practical laboratory experience in the PSCC. The lectures, supplemented by practice, problems sets, slides, and video tapes, provide the fundamentals needed to understand the techniques and instrumentation involved in this powerful analytical tool. No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course. Some experience in HPLC or introductory course work in organic chemistry is desirable but not necessary.

Key Topics of This Dynamic Course
  Basic instrumentation for HPLC
  Detectors: UV/VIS, photo diode array, mass spectrometer, fluorescence
  Column selection and optimization
  Qualitative and quantitative analysis
  Troubleshooting HPLC systems

How You'll Benefit from This Course
  Learn about basic instrumentation for HPLC
  Learn about detectors: UV/VIS and PDA
  Become familiar with column selection and optimization
  Perform qualitative and quantitative analysis
  Understand how to troubleshoot HPLC systems

Course Topics
  HPLC Overview
    Introduction to HPLC, instrumentation overview, detector details and laboratory:
    basic HPLC hardware

  Separation Fundamentals and Mobile Phase
    Practical HPLC theory, important HPLC parameters, the mobile phase and laboratory:
    mobile phase strength flow rate and temperature

 Separation Modes and Columns
   Reversed phase, normal phase, ion exchange, size exchange and laboratories:
   pH effects in reversed phase and column dimensions.

 Quantitative Analysis and Troubleshooting
   Qualitative and quantitative analysis, hardware troubleshooting, separation
   troubleshooting and laboratory: internal vs. external standard; hardware troubleshooting

 Gradients and Method Development
   Gradients; fundamentals of method development; laboratory:
   develop a method from a completely unknown mixture